Pair Name	Polydatin, 2-Deoxy-d-glucose
Disease Info	[ICD-11: 2C60]	Breast cancer		Investigative
Biological Phenomena	Inhibition-->Glycolysis
Gene Regulation	Down-regulation	Expression	AKT1	hsa207
	Up-regulation	Expression	BAX	hsa581
	Down-regulation	Expression	BCL2	hsa596
	Down-regulation	Expression	HIF1A	hsa3091
	Down-regulation	Expression	HK2	hsa3099
	Down-regulation	Expression	MMP2	hsa4313
	Down-regulation	Expression	MMP9	hsa4318
	Up-regulation	Expression	PARP1	hsa142
	Down-regulation	Expression	PIK3CA	hsa5290
	Down-regulation	Expression	ROS1	hsa6098
	Down-regulation	Expression	VEGFA	hsa7422
In Vitro Model	4T1	Malignant neoplasms of the mouse mammary gland	Mus musculus (Mouse)	CVCL_0125
	MCF-7	Invasive breast carcinoma of no special type	Homo sapiens (Human)	CVCL_0031
In Vivo Model	Approximately 2.0×10⁷ 4T1 cells without any contamination were harvested and suspended in 100 μL of PBS, then subcutaneously injected into the mouse's fourth breast pad.
Result	Our study demonstrates that PD synergised with 2-DG to enhance its anti-cancer efficacy by inhibiting the ROS/PI3K/AKT/HIF-1α/HK2 signalling axis, providing a potential anti-cancer strategy.